Genetic modification of essential fatty acids biosynthesis inHansenula polymorpha

Creation of Hansenula polymorpha fatty acid auxotrophic mutants for genetic study of fatty acid biosynthesis

Three groups of Hansenula polymorpha fatty acid auxotrophic mutants were screened by their fatty acid requirement for growth. The mutants are saturated fatty acid auxotrophic mutants (Sfa), monounsaturated fatty acid auxotrophic mutants (Mfa) and polyunsaturated fatty acid auxotrophic mutants (Pfa) which required saturated fatty acids, monounsaturated fatty acids and polyunsaturated fatty acids...

Dietary fatty acids in an era of genetic modification.

Consequences of Essential Fatty Acids

Essential fatty acids (EFA) are nutrients that form an amazingly large array of bioactive mediators that act on a large family of selective receptors. Nearly every cell and tissue in the human body expresses at least one of these receptors, allowing EFA-based signaling to influence nearly every aspect of human physiology. In this way, the health consequences of specific gene-environment interac...

Metabolism of Essential Fatty Acids

The fat deficiency disease in the rat (I), dog (2), and chick (3) is characterized in part by sharply increased concentrations of one or more trienoic acids in the tissues, as indicated by alkali isomerization analyses. Indeed, the increase in conjugatable triene in the mitochondrial fatty acids may be the first noticeable symptom of the onset of fat deficiency (4). The major triene component o...

Biological modification of the fatty acid group in an emulsan by supplementing fatty acids under conditions inhibiting fatty acid biosynthesis.

When the concentration of the antibiotic cerulenin was increased up to 3.0 mg/l in medium containing ethanol as a carbon source, the specific growth rate of Acinetobacter calcoaceticus and the fatty acid content of the emulsan decreased from 0.179 h(-1) and 13.9% to 0.015 h(-1) and 3.4%, respectively. The emulsifying activity in medium containing cerulenin decreased with increasing cerulenin co...